(Kahe cyptley, fcm) he mea kelepona e helu ana i ka uluʻana o nā mea i loaʻa i nā hōʻailona kelepona. ʻO ia kahiʻenehana kiʻekiʻe-Technology i kūkuluʻia ma luna o ka nānāʻana a me ka hoʻokaʻawaleʻana i nā'āpana hoʻokahi. Hiki iā ia ke ana a hoʻopaʻa i ka nui, kūlike kūloko,ʻo RNA,ʻo nā mea kūʻai aku a me nā mea kūʻai aku a me nā mea kūʻai aku o kēia mau helu.
ʻO ke kaheʻana o ka cytomter ma mua o nā'āpanaʻelima:
1 kahe wai a me keʻano o ka'ōnaehana fludics
ʻO 2 Laser māmā Source
3 Pūnaehana kiko
ʻO 4 uila, waiho ana, e hōʻike ana, hōʻike a me ka'ōnaehana loiloi
5 kelepona hoʻonohonoho hoʻonohonoho
I waena o lākou,ʻo Laser hoihoi i loko o ka Source Light Light Light a me Beham State Systemʻo ia ka ana nui o nā hōʻailona fluerescence ʻO ka ikaika o ka mālamalama o ka mālamalama a me ka manawa hoʻolaha e pili ana i ka uluʻana o ka nui o ka pākeke. ʻO Laser kahi kumu kukui māmā e hiki ke hāʻawi i ke kiʻekiʻe hoʻokahi, kiʻekiʻe-kiʻekiʻe, a me keʻano kiʻekiʻe kiʻekiʻe. ʻO ia ka mea hoʻonaninani maikaʻi loa e hoʻokō ai i kēia mau koi.
Aia he mau lens cylindrical ma waena o ke kumu laser a me ke keʻena o ke kahe. ʻO kēia mau lenses i kākoʻo i kahi pahu laser me kahi'āpana cross-racular i hoʻopiliʻia mai ka papaʻo Laser i kahi'āpana elliptical i kahi'āpana liʻiliʻi (22 μ6 × 66 μ 6. Ua māheleʻia ka ikaikaʻo Laser ma loko o kēia Boam elliptical e like me ka māhele maʻamau, e hōʻoia ana i ka hoʻonuiʻana i kaʻike i kaʻaoʻao o kaʻaoʻao. Ma kaʻaoʻao'ē aʻe,ʻo ka waihona ma kaʻaoʻao o nā Levi, nā pou, a me nā kānana e puni i waena o ke keʻenaʻauʻau moana.
ʻO ka'ōnaehana optical ma mua o ke keʻena o ke keʻena kahe o ke keʻena o ke keʻena o kahi lens a me ka pinhole. ʻO ka hana nui o nā lens a me nā pinhole (maʻamauʻelua mau lenses a me kahi pinhole e hoʻopili ai i kahi'āpana o ka laser i kahi'āpana o keʻano. Hāʻawi kēia i ka ikehu laser e like me ka māhele maʻamau, e hōʻoia ana i ka ikaika e hiki mai ana i ka nui o nā cell ma waena o nā'āpana.
ʻEkolu mau mea nui o nā kānana:
1:ʻO nā kānana lōʻihi
2:ʻO kāna kānana pōkole (SPF) - eʻae wale i ka māmā me nā mea i hala ma lalo o kahi waiwai kiko'ī e hele ai.
3:ʻO nā kānana Bandspus (bpf)
Hiki i nā hui like o nā kānana ke alakaʻi i nā hōʻailona fluerescence i nā hōʻailona o nā mea likeʻole i nā puka kiʻi kiʻi likeʻole No ka hoʻohālikelikeʻana, nā kānana e loaʻa ai ke nānāʻana i ke aniani'ōmaʻomaʻo (Fitc) i mua o PMT he LPF550 a me BPF525. Ua hoʻohanaʻia nā kānana i hoʻohanaʻia eʻike i ka fluor-red fluorescence (pe) i mua o ka pmt he lpf600 a me bpf575. ʻO nā kānana no ka nānāʻana i kaʻulaʻula ulaula (cy5) i mua o ka pmt he lpf650 a me bpf675.
Hoʻohana nuiʻia ke kaheʻana o ka cytometry no ka hoʻokaʻawaleʻana. Me ka holomua o kaʻenehana kamepiula,ʻo ka hoʻomohalaʻana o ka immunology a me keʻano o kaʻenehanaʻo Mononclos ʻO kēia mau noi noi e pili ana i ka nānāʻana i ka cell dynamics
Post Times: Sep-21-2023
