(Flow cytometry , FCM ) he mea hoʻoponopono kelepona e ana i ka ikaika o ka fluorescence o nā hōʻailona cell stained. He ʻenehana kiʻekiʻe ia i hoʻomohala ʻia ma muli o ka nānā ʻana a me ka hoʻokaʻawale ʻana i nā cell hoʻokahi. Hiki iā ia ke ana wikiwiki a hoʻokaʻawale i ka nui, ka hale i loko, ka DNA, RNA, nā protein, nā antigens a me nā waiwai kino a i ʻole kemika o nā cell, a hiki ke hoʻokumu ʻia ma ka hōʻiliʻili ʻana o kēia mau papa.
ʻO Flow cytometer ka nui o nā ʻāpana ʻelima:
1 Keʻena kahe a me ka ʻōnaehana fluidics
2 ʻO ke kumu kukui laser a me ka ʻōnaehana hana kukuna
3 Pūnaehana Optical
4 Electronics, mālama, hōʻike a me ka ʻōnaehana loiloi
5 Pūnaehana helu kelepona
Ma waena o lākou, ʻo ka hoʻoulu ʻana o ka laser i ke kumu kukui laser a me ka ʻōnaehana hana kukuna ke ana nui o nā hōʻailona fluorescence i ka cytometry kahe. ʻO ka ikaika o ke kukui hoʻoulu a me ka manawa hoʻolaha e pili ana i ka ikaika o ka hōʻailona fluorescence. ʻO Laser kahi kumu kukui koʻikoʻi e hiki ke hāʻawi i ka lōʻihi o ka nalu hoʻokahi, ka ikaika kiʻekiʻe, a me ka hoʻomālamalama kiʻekiʻe. ʻO ia ke kumu kukui excitation kūpono e hoʻokō i kēia mau koi.
ʻElua mau lens cylindrical ma waena o ke kumu laser a me ke keʻena kahe. Hoʻopili kēia mau lens i kahi kukuna laser me kahi ʻāpana keʻa pōʻai i hoʻokuʻu ʻia mai ke kumu laser i loko o kahi kukui elliptical me kahi ʻāpana liʻiliʻi (22 μm × 66 μm). Hoʻokaʻawale ʻia ka ikehu laser i loko o kēia beam elliptical e like me ka puʻunaue maʻamau, e hōʻoia ana i ka ikaika o ka hoʻomālamalama mau ʻana no nā cell e hele ana i ka wahi ʻike laser. Ma ka ʻaoʻao ʻē aʻe, aia ka ʻōnaehana optical i nā ʻāpana he nui o nā lens, pinholes, a me nā kānana, hiki ke hoʻokaʻawale ʻia i ʻelua pūʻulu: i luna a i lalo o ke keʻena kahe.
ʻO ka ʻōnaehana optical i mua o ke keʻena kahe he lens a me ka pinhole. ʻO ka hana nui o ka lens a me ka pinhole (ʻo ka maʻamau ʻelua mau lens a me kahi pinhole) ʻo ia ka hoʻopaʻa ʻana i ka kukuna laser me kahi ʻāpana keʻa pōʻai i hoʻokuʻu ʻia e ke kumu laser i loko o kahi kukui elliptical me kahi ʻāpana liʻiliʻi. Hāʻawi kēia i ka ikehu laser e like me ka puʻunaue maʻamau, e hōʻoiaʻiʻo ana i ka ikaika o ka hoʻomālamalama mau ʻana no nā cell ma o ka wahi ʻike laser a me ka hōʻemi ʻana i ka hoʻopilikia ʻana mai ka mālamalama hele.
ʻEkolu ʻano nui o nā kānana:
1: Long pass filter (LPF) - ʻae wale i ka mālamalama me nā lōʻihi hawewe i ʻoi aku ka kiʻekiʻe ma mua o kahi waiwai kikoʻī e hele ai.
2: kānana pōkole pōkole (SPF) - ʻae wale i ka mālamalama me nā lōʻihi hawewe ma lalo o kahi waiwai kikoʻī e hele i loko.
3: Kānana Bandpass (BPF) - ʻae wale i ka mālamalama i kahi laulā hawewe kikoʻī e hele i loko.
Hiki i nā hui like ʻole o nā kānana ke kuhikuhi i nā hōʻailona fluorescence i nā lōʻihi nalu like ʻole i nā paipu photomultiplier pākahi (PMT). No ka laʻana, ʻo nā kānana no ka ʻike ʻana i ka fluorescence ʻōmaʻomaʻo (FITC) i mua o PMT ʻo LPF550 a me BPF525. ʻO nā kānana i hoʻohana ʻia e ʻike i ka fluorescence ʻalani-ʻulaʻula (PE) i mua o ka PMT ʻo LPF600 a me BPF575. ʻO nā kānana no ka ʻike ʻana i ka fluorescence ʻulaʻula (CY5) i mua o ka PMT ʻo LPF650 a me BPF675.
Hoʻohana nui ʻia ka Flow cytometry no ka hoʻokaʻawale ʻana i nā cell. Me ka holomua o ka ʻenehana lolouila, ka hoʻomohala ʻana o ka immunology a me ka hana ʻana o ka ʻenehana antibody monoclonal, ʻo kāna mau noi i ka biology, lāʻau lapaʻau, lāʻau lapaʻau a me nā kula ʻē aʻe e hoʻonui nui ʻia. Hoʻopili kēia mau noi i ka loiloi cell dynamics, cell apoptosis, cell typing, tumor diagnostic, drug efficacy analysis, etc.
Ka manawa hoʻouna: Sep-21-2023
